Human LGALS1 Recombinant Protein (RPPB0273)
- SKU:
- RPPB0273
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P09382
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human LGALS1 Recombinant Protein |
| Product Code: | RPPB0273 |
| Size: | 50µg |
| Species: | Human |
| Target: | LGALS1 |
| Synonyms: | Galectin-1, GAL1, GAL-1, Lectin galactoside-binding soluble 1, Beta-galactoside- binding lectin L-14-I, Lactose-binding lectin 1, S-Lac lectin 1, Galaptin, 14 kDa lectin, HPL, HBL, Putative MAPK-activating protein PM12, GBP, DKFZp686E23103. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The Galectin-1 protein was lyophilized from a concentrated (1mg/ml) containing 10mM sodium phosphate, pH-7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized LGALS1 in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Galectin-1 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MACGLVASNL NLKPGECLRV RGEVAPDAKS FVLNLGKDSN NLCLHFNPRF NAHGDANTIV CNSKDGGAWG TEQREAVFPF QPGSVAEVCI TFDQANLTVK LPDGYEFKFP NRLNLEAINY MAADGDFKIK CVAFD |
| Biological Activity: | The activity of Human Galectin-1 which is determined by the ability to induce chemotaxis of human THP-1 cells is detectable starting at 100ng/ml, corresponding to a specific activity of 1.0x104 units/mg. |
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 is an autocrine negative growth factor that regulates cell proliferation. Galectin-1 regulates cell apoptosis and cell differentiation. Galectin-1 binds CD45, CD3 and CD4 & inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of lyn kinase. Galectin-1 and its ligands are one of the master regulators of immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation and allergies as well as host�pathogen interactions. Galectin-1 expression or overexpression in tumors and/or the tissue surrounding them must be considered as a sign of the malignant tumor progression that is often related to the long-range dissemination of tumoral cells (metastasis), to their dissemination into the surrounding normal tissue, and to tumor immune-escape. Galectin-1 in its oxidized form plays a number of important roles in the regeneration of the central nervous system after injury. The targeted overexpression (or delivery) of Galectin-1 should be considered as a method of choice for the treatment of some kinds of inflammation-related diseases, neurodegenerative pathologies and muscular dystrophies. In contrast, the targeted inhibition of Galectin-1 expression is what should be developed for therapeutic applications against cancer progression. Galectin-1 is thus a promising molecular target for the development of new and original therapeutic tools. There is 88% homology between the human and mouse galectin-1.
LGALS1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 135 amino acids and having a molecular mass of 14.7kDa.The LGALS1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. |
| UniProt Protein Details: | Protein type:Cell adhesion Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; intracellular; nucleus Molecular Function:signal transducer activity; protein binding; protein homodimerization activity; lactose binding; galactoside binding; laminin binding; glycoprotein binding Biological Process: regulation of apoptosis; response to drug; myoblast differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; plasma cell differentiation; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress |
| NCBI Summary: | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09382 |
| NCBI GenInfo Identifier: | 126155 |
| NCBI Gene ID: | 3956 |
| NCBI Accession: | P09382.2 |
| UniProt Secondary Accession: | P09382,Q9UDK5, B2R5E8, |
| UniProt Related Accession: | P09382 |
| Molecular Weight: | 14,716 Da |
| NCBI Full Name: | Galectin-1 |
| NCBI Synonym Full Names: | lectin, galactoside-binding, soluble, 1 |
| NCBI Official Symbol: | LGALS1�� |
| NCBI Official Synonym Symbols: | GBP; GAL1�� |
| NCBI Protein Information: | galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa |
| UniProt Protein Name: | Galectin-1 |
| UniProt Synonym Protein Names: | 14 kDa laminin-binding protein; HLBP14; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1 |
| Protein Family: | Galectin |
| UniProt Gene Name: | LGALS1�� |
| UniProt Entry Name: | LEG1_HUMAN |
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