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Human Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) ELISA Kit

SKU:
HUDL01717
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8N6C8
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD85e,HM31,HM43,ILT6,LIR4,E3,CD85 antigen-like family member E,Immunoglobulin-like transcript 6,Leukocyte immunoglobulin-like receptor 4
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) ELISA Kit

The Human Leukocyte Immunoglobulin-Like Receptor Subfamily A Member 3 (LILRA3) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of LILRA3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and reproducibility, ensuring consistent and dependable results for a variety of research applications.LILRA3 is a key protein involved in immune regulation and has been linked to various autoimmune diseases and inflammatory conditions.

The detection of LILRA3 levels can provide valuable insights into the immune response and help in the development of novel therapeutic strategies for treating such disorders.With its exceptional performance and versatility, the LILRA3 ELISA Kit is an indispensable tool for scientists and researchers studying immunology, autoimmune diseases, and inflammation. Invest in this kit today to unlock new possibilities in your research endeavors.

Product Name:Human Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3) ELISA Kit
Product Code:HUDL01717
Size:96 Assays
Target:LILRA3
Synonyms:CD85e, HM31, HM43, ILT6, LIR4, E3, CD85 antigen-like family member E, Immunoglobulin-like transcript 6, Leukocyte immunoglobulin-like receptor 4
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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