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Human Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) ELISA Kit

SKU:
HUDL01761
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q32MZ4
Sensitivity:
0.127ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
GCF2,FLAP-1,FLIIAP1,GCF-2,HUFI-1,TRIP,GC-Binding Factor 2,TAR RNA-interacting protein
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) ELISA Kit

The Human Leucine Rich Repeat in FLII Interacting Protein 1 (LRRFIP1) ELISA Kit is specifically designed for the precise detection of LRRFIP1 levels in human serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.LRRFIP1 is a critical protein that interacts with FLII and plays a role in various cellular processes, including immune response, apoptosis, and gene regulation. Dysregulation of LRRFIP1 has been linked to inflammatory diseases, autoimmune disorders, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

With the Human LRRFIP1 ELISA Kit, researchers can confidently measure LRRFIP1 levels in biological samples, providing valuable insights into its function and potential implications in disease pathology. This advanced kit is a powerful tool for advancing scientific discoveries and exploring new avenues for targeted treatments.

Product Name:Human Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1) ELISA Kit
Product Code:HUDL01761
Size:96 Assays
Target:LRRFIP1
Synonyms:GCF2, FLAP-1, FLIIAP1, GCF-2, HUFI-1, TRIP, GC-Binding Factor 2, TAR RNA-interacting protein
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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