The Human LEPR (Leptin Receptor) ELISA Kit by Assay Genie is expertly crafted for the precise quantitative measurement of Leptin Receptor (LEPR) levels in a variety of biological samples. LEPR, as a key player in the regulation of energy balance and metabolism, is integrally involved in modulation of appetite, energy expenditure, and adipogenesis. Understanding the intricacies of LEPR signaling pathways is crucial in unraveling the mechanisms underpinning metabolic disorders and obesity-related complications.
Our LEPR ELISA Kit stands out for its exceptional sensitivity and specificity, guaranteeing accurate and reproducible results in your research endeavors. With stringent quality control measures in place, this kit assures robust performance, making it an ideal choice for conducting cutting-edge research studies. Trust Assay Genie's LEPR ELISA Kit for reliable and insightful data insights that drive scientific discovery.
Product Name:
Human LEPR (Leptin Receptor) ELISA Kit
SKU:
AEES00174
Target:
Human LEPR (Leptin Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LEPR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEPR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEPR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LEPR. You can calculate the concentration of Human LEPR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
85-99
97-112
Average (%)
101
92
103
1:4
Range (%)
96-112
88-99
97-111
Average (%)
102
94
104
1:8
Range (%)
90-108
84-96
92-104
Average (%)
98
90
99
1:16
Range (%)
97-112
85-100
96-112
Average (%)
104
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
101
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
87-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.91
4.4
14.18
1.81
4.63
15.49
Standard deviation
0.12
0.26
0.63
0.11
0.2
0.57
C V (%)
6.42
5.96
4.47
6.04
4.4
3.69
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LEPR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LEPR in samples. No significant cross-reactivity or interference between Human LEPR and analogues was observed.
