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Human Lemur Tyrosine Kinase 3 (LMTK3) ELISA Kit

SKU:
HUDL01731
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q96Q04
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LMR3,TYKLM3,Serine/threonine-protein kinase LMTK3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Lemur Tyrosine Kinase 3 (LMTK3) ELISA Kit

The Human Lemur Tyrosine Kinase 3 (LMTK3) ELISA Kit is a powerful tool for accurately measuring LMTK3 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers precise and reliable results, making it suitable for a variety of research applications.LMTK3 is a key protein involved in various cellular processes, including cell growth, survival, and migration. Dysregulation of LMTK3 has been implicated in cancer progression, making it a valuable biomarker for studying cancer biology and potentially identifying new therapeutic targets.

Overall, the Human LMTK3 ELISA Kit offers researchers a valuable tool for investigating the role of LMTK3 in health and disease, ultimately contributing to a better understanding of cancer biology and potentially leading to the development of novel treatment strategies.

Product Name:Human Lemur Tyrosine Kinase 3 (LMTK3) ELISA Kit
Product Code:HUDL01731
Size:96 Assays
Target:LMTK3
Synonyms:LMR3, TYKLM3, Serine/threonine-protein kinase LMTK3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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