The Human LDHA (Lactate Dehydrogenase A) CLIA Kit is a reliable assay designed for the quantitative detection of LDHA levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.LDHA is a key enzyme involved in the conversion of lactate to pyruvate, playing a critical role in cellular metabolism and energy production. Abnormal LDHA levels have been associated with various diseases such as cancer, cardiovascular disorders, and metabolic diseases, making it a valuable biomarker for understanding these conditions and developing potential treatments.
With its user-friendly protocol and robust performance, the Human LDHA CLIA Kit is an essential tool for researchers studying the role of LDHA in health and disease. Get reliable data and valuable insights with this advanced assay kit from Assay Genie.
Product Name:
Human LDHA (Lactate Dehydrogenase A) CLIA Kit
SKU:
HUES00382
Target:
Human LDHA (Lactate Dehydrogenase A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LDHA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LDHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LDHA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LDHA. You can calculate the concentration of Human LDHA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-118
102-119
91-105
Average (%)
108
108
98
1:4
Range (%)
93-108
96-112
99-113
Average (%)
99
104
104
1:8
Range (%)
88-103
102-116
98-113
Average (%)
96
109
104
1:16
Range (%)
92-103
86-100
99-114
Average (%)
98
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
97-111
105
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.3
4.76
0.46
1.24
5.18
Standard deviation
0.05
0.09
0.5
0.06
0.1
0.54
C V (%)
10.87
6.92
10.5
13.04
8.06
10.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LDHA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LDHA in samples. No significant cross-reactivity or interference between Human LDHA and analogues was observed.
