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Human LCMT1 Recombinant Protein (RPPB1886)

SKU:
RPPB1886
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UIC8
Research Area:
Enzymes
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Description

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Product Name:Human LCMT1 Recombinant Protein
Product Code:RPPB1886
Size:20µg
Species:Human
Target:LCMT1
Synonyms:Leucine carboxyl methyltransferase 1, LCMT1, LCMT, PPMT1, CGI-68.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The LCMT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.15M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMATRQR ESSITSCCST SSCDADDEGV RGTCEDASLC KRFAVSIGYW HDPYIQHFVR LSKERKAPEI NRGYFARVHG VSQLIKAFLR KTECHCQIVN LGAGMDTTFW RLKDEDLLPS KYFEVDFPMI VTRKLHSIKC KPPLSSPILE LHSEDTLQMD GHILDSKRYA VIGADLRDLS ELEEKLKKCN MNTQLPTLLI AECVLVYMTP EQSANLLKWA ANSFERAMFI NYEQVNMGDR FGQIMIENLR RRQCDLAGVE TCKSLESQKE RLLSNGWETA SAVDMMELYN RLPRAEVSRI ESLEFLDEME LLEQLMRHYC LCWATKGGNE LGLKEITY

LCMT1 is a member of the LCMT family, methyltransferase superfamily. LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A to form alpha-leucine ester residues.

LCMT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 358 amino acids (1-334) and having a molecular mass of 41kDa.LCMT1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:LCMT1: Methylates the carboxyl group of the C-terminal leucine residue of protein phosphatase 2A catalytic subunits to form alpha-leucine ester residues. Belongs to the methyltransferase superfamily. LCMT family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - histidine; EC 2.1.1.233; Lipid Metabolism - androgen and estrogen; Methyltransferase; Other Amino Acids Metabolism - selenoamino acidChromosomal Location of Human Ortholog: 16p12.1Cellular Component: cytosolMolecular Function: protein binding; C-terminal protein carboxyl methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activityBiological Process: regulation of apoptosis; protein amino acid methylation; C-terminal protein amino acid methylation; protein modification process; negative regulation of protein complex assembly
UniProt Protein Details:
NCBI Summary:LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]).[supplied by OMIM, Mar 2008]
UniProt Code:Q9UIC8
NCBI GenInfo Identifier:74048545
NCBI Gene ID:51451
NCBI Accession:NP_001027563.1
UniProt Secondary Accession:Q9UIC8,Q53FC5, Q96CI5, Q9H6I9, Q9NTG4, Q9Y378, A6NL89 A8K770
UniProt Related Accession:Q9UIC8
Molecular Weight:32,172 Da
NCBI Full Name:leucine carboxyl methyltransferase 1 isoform b
NCBI Synonym Full Names:leucine carboxyl methyltransferase 1
NCBI Official Symbol:LCMT1��
NCBI Official Synonym Symbols:LCMT; PPMT1; CGI-68��
NCBI Protein Information:leucine carboxyl methyltransferase 1; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1; protein phosphatase methyltransferase 1; protein-leucine O-methyltransferase
UniProt Protein Name:Leucine carboxyl methyltransferase 1
UniProt Synonym Protein Names:Protein-leucine O-methyltransferase; [Phosphatase 2A protein]-leucine-carboxy methyltransferase 1
Protein Family:Leucine carboxyl methyltransferase
UniProt Gene Name:LCMT1��
UniProt Entry Name:LCMT1_HUMAN

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