Human LAT (Linker For Activation of T-cell) CLIA Kit
The Human LAT (Linker for Activation of T cells) CLIA Kit is a powerful tool for measuring LAT levels in human samples with high accuracy and sensitivity. LAT is a key protein involved in T cell activation, enhancing immune responses and regulating cell signaling pathways.This kit provides researchers with a reliable and reproducible method for studying T cell function and immune response in various physiological and pathological conditions. Its versatility and ease of use make it suitable for a wide range of research applications, from basic research to drug development.
By measuring LAT levels, researchers can gain valuable insights into the immune system's response to infections, autoimmune diseases, and cancer. This information can guide the development of targeted therapies for these conditions, ultimately improving patient outcomes.Overall, the Human LAT CLIA Kit offers researchers a comprehensive solution for studying T cell activation and immune response, paving the way for new discoveries in immunology and immunotherapy.
Product Name:
Human LAT (Linker For Activation of T-cell) CLIA Kit
SKU:
HUES00568
Target:
Human LAT (Linker For Activation of T-cell)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LAT. You can calculate the concentration of Human LAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
90-102
97-110
Average (%)
94
97
104
1:4
Range (%)
87-102
101-117
100-116
Average (%)
94
109
106
1:8
Range (%)
86-98
92-103
101-114
Average (%)
91
98
106
1:16
Range (%)
100-111
97-110
89-105
Average (%)
106
103
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
106
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
199.35
458.22
1759.83
196.53
442.23
1641.21
Standard deviation
18.3
43.03
137.79
23.92
43.82
135.24
C V (%)
9.18
9.39
7.83
12.17
9.91
8.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LAT in samples. No significant cross-reactivity or interference between Human LAT and analogues was observed.
