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Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit

SKU:
HUDL01730
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28065
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PSMB9,Proteasome(Prosome,Macropain)Subunit Beta Type 9,Macropain chain 7,Multicatalytic endopeptidase complex chain 7,Really interesting new gene 12 protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit

The Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit is specially designed for the precise measurement of LMP2 levels in human samples, including serum, plasma, and cell culture supernatants. With its advanced sensitivity and specificity, this kit guarantees accurate and consistent results, making it an invaluable tool for a variety of research purposes.LMP2 is a multifunctional peptidase that plays a vital role in protein degradation and immune response modulation. Dysregulation of LMP2 has been implicated in various diseases, including autoimmune disorders, inflammatory conditions, and cancer.

By measuring LMP2 levels, researchers can gain insight into disease pathogenesis and potential therapeutic targets.Overall, the Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit offers researchers a reliable and efficient solution for studying the role of LMP2 in health and disease, paving the way for advancements in diagnostics and treatment strategies.

Product Name:Human Large Multifunctional Peptidase 2 (LMP2) ELISA Kit
Product Code:HUDL01730
Size:96 Assays
Target:LMP2
Synonyms:PSMB9, Proteasome(Prosome,Macropain)Subunit Beta Type 9, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Really interesting new gene 12 protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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