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Human Laminin Gamma 2 (LAMC2) ELISA Kit

SKU:
HUDL01678
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13753
Sensitivity:
0.26ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
B2T,EBR2,EBR2A,LAMB2T,LAMNB2,Kalinin/nicein/epiligrin 100 kDa subunit,Cell-scattering factor 140 kDa subunit,Ladsin 140 kDa,Large adhesive scatter factor 140 kDa
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Laminin Gamma 2 (LAMC2) ELISA Kit

The Human Laminin gamma 2 (LAMC2) ELISA Kit is specifically designed for the quantitative measurement of LAMC2 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and precise, providing reliable and consistent results for a variety of research applications.Laminin gamma 2 is an important component of the extracellular matrix, playing a key role in cell adhesion, migration, and tissue development. Dysregulation of LAMC2 has been linked to various diseases, including cancer, muscular dystrophy, and skin disorders.

By accurately measuring LAMC2 levels, researchers can gain valuable insights into disease pathology and potential therapeutic interventions.The Human Laminin gamma 2 (LAMC2) ELISA Kit is a valuable tool for investigating the role of LAMC2 in disease progression and developing novel treatment strategies. Its high sensitivity and specificity make it an essential asset for researchers in the fields of oncology, pathology, and regenerative medicine.

Product Name:Human Laminin Gamma 2 (LAMC2) ELISA Kit
Product Code:HUDL01678
Size:96 Assays
Target:LAMC2
Synonyms:B2T, EBR2, EBR2A, LAMB2T, LAMNB2, Kalinin/nicein/epiligrin 100 kDa subunit, Cell-scattering factor 140 kDa subunit, Ladsin 140 kDa, Large adhesive scatter factor 140 kDa
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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