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Human Laminin Gamma 1 (LAMC1) ELISA Kit

SKU:
HUDL01677
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11047
Sensitivity:
12.1pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
LAM-C1,LAMB2,S-laminin subunit gamma,Laminin B2 chain
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Laminin Gamma 1 (LAMC1) ELISA Kit

The Human Laminin Gamma 1 (LAMC1) ELISA Kit is specifically designed for the precise measurement of Laminin Gamma 1 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Laminin Gamma 1 is a key component of laminin proteins, critical for cell adhesion, migration, and tissue organization.

Dysregulation of Laminin Gamma 1 has been implicated in various diseases, including cancer, muscular dystrophy, and kidney disorders. Therefore, measuring Laminin Gamma 1 levels can provide valuable insights into disease mechanisms and potential therapeutic strategies.With its reliable performance and broad utility, the Human Laminin Gamma 1 ELISA Kit is an indispensable tool for researchers studying the role of Laminin Gamma 1 in health and disease.

Product Name:Human Laminin Gamma 1 (LAMC1) ELISA Kit
Product Code:HUDL01677
Size:96 Assays
Target:LAMC1
Synonyms:LAM-C1, LAMB2, S-laminin subunit gamma, Laminin B2 chain
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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