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Human Laminin Beta 2 (LAMb2) ELISA Kit

SKU:
HUDL01675
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P55268
Sensitivity:
0.33ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
LAM-B2,LAMS,Laminin S,Laminin B1s chain,S-laminin subunit beta
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Laminin Beta 2 (LAMb2) ELISA Kit

The Human Laminin Beta-2 (LAMB2) ELISA Kit is specifically designed for the precise and accurate detection of LAMB2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and trustworthy results for a variety of research purposes.Laminin Beta-2 is a vital protein that plays a crucial role in cell adhesion and migration, making it essential for tissue development and maintenance.

Dysregulation of LAMB2 has been linked to various diseases, including kidney disorders and certain types of cancer, highlighting its significance as a biomarker for disease research and therapeutic development.With its user-friendly format and robust performance, the Human Laminin Beta-2 (LAMB2) ELISA Kit is a valuable tool for scientists and researchers looking to investigate the role of LAMB2 in health and disease.

Product Name:Human Laminin Beta 2 (LAMb2) ELISA Kit
Product Code:HUDL01675
Size:96 Assays
Target:LAMb2
Synonyms:LAM-B2, LAMS, Laminin S, Laminin B1s chain, S-laminin subunit beta
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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