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Human Lamin A/C (LMNA) ELISA Kit

SKU:
HUDL01727
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P02545
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CDCD1,CMD1A,CMT2B1,EMD2,FPL,FPLD,HGPS,IDC,LDP1,LFP,LGMD1B,LMN1,LMNC,PRO1,Cardiomyopathy Dilated 1A,Limb Girdle Muscular Dystrophy 1B,Progeria 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Lamin A/C (LMNA) ELISA Kit

The Human Lamin A/C (LMNA) ELISA Kit is a reliable and accurate tool for measuring levels of lamin A/C in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Lamin A/C is an important protein that plays a key role in maintaining the structural integrity of the cell nucleus. Mutations in the LMNA gene have been associated with a range of diseases, including muscular dystrophies, cardiomyopathies, and progeroid syndromes.

Therefore, measuring levels of lamin A/C can provide valuable insights into the pathogenesis of these diseases and aid in the development of potential therapies.Overall, the Human Lamin A/C (LMNA) ELISA Kit is a valuable tool for researchers studying cellular structural biology, disease mechanisms, and potential therapeutic interventions.

Product Name:Human Lamin A/C (LMNA) ELISA Kit
Product Code:HUDL01727
Size:96 Assays
Target:LMNA
Synonyms:CDCD1, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, PRO1, Cardiomyopathy Dilated 1A, Limb Girdle Muscular Dystrophy 1B, Progeria 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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