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Human Lactate Dehydrogenase D (LDHD) ELISA Kit

SKU:
HUDL01697
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q86WU2
Sensitivity:
0.59ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
LDH-D,DLD,Probable D-lactate dehydrogenase, mitochondrial
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Lactate Dehydrogenase D (LDHD) ELISA Kit

The Human Lactate Dehydrogenase D (LDHD) ELISA Kit is a reliable and accurate tool for measuring LDHD levels in human samples. LDHD is an enzyme that plays a key role in the metabolism of lactate, making it essential for energy production in cells. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.LDHD levels can serve as important biomarkers for various health conditions, including tissue injury, liver disease, and certain types of cancer.

By measuring LDHD levels, researchers can gain valuable insights into disease progression and treatment efficacy. This ELISA kit is a valuable resource for studying the role of LDHD in health and disease, as well as for developing potential therapeutic interventions.

Product Name:Human Lactate Dehydrogenase D (LDHD) ELISA Kit
Product Code:HUDL01697
Size:96 Assays
Target:LDHD
Synonyms:LDH-D, DLD, Probable D-lactate dehydrogenase, mitochondrial
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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