The Human KRAS (Kirsten rat sarcoma viral oncogene homolog) ELISA Kit is a cutting-edge assay designed for the precise and quantitative measurement of KRAS protein levels in human samples. KRAS, a critical oncogene linked to various cancers, including colorectal cancer and lung cancer, plays a key role in cell signaling pathways that regulate cell growth and proliferation. Understanding the expression levels of KRAS is crucial for unraveling the molecular processes underlying cancer development and progression. With exceptional sensitivity and specificity, the Human KRAS ELISA Kit enables researchers to accurately quantify KRAS protein levels, providing valuable insights into oncogenic signaling pathways and potential therapeutic targets.
By facilitating the precise measurement of KRAS, this ELISA kit empowers researchers to investigate the role of KRAS in cancer biology, drug resistance, and personalized treatment strategies, ultimately advancing cancer research and precision medicine. Manufactured under stringent quality control standards, the Human KRAS ELISA Kit offers robust performance and reproducibility, ensuring accurate and reliable results in cancer research studies. Researchers exploring KRAS-mediated oncogenesis and targeted therapy development can benefit from this user-friendly kit to enhance their investigations and contribute to the understanding of KRAS-associated malignancies.
Product Name:
Human KRAS ELISA Kit
SKU:
AEES00669
Target:
Human KRAS
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KRAS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KRAS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KRAS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KRAS. You can calculate the concentration of Human KRAS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
95-108
98-112
Average (%)
97
101
106
1:4
Range (%)
89-100
80-91
82-94
Average (%)
94
86
88
1:8
Range (%)
93-106
81-92
88-99
Average (%)
100
86
94
1:16
Range (%)
93-107
80-94
83-95
Average (%)
100
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.21
8.44
1.0
2.19
8.62
Standard deviation
0.06
0.1
0.32
0.07
0.1
0.36
C V (%)
6.32
4.52
3.79
7.0
4.57
4.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human KRAS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KRAS in samples. No significant cross-reactivity or interference between Human KRAS and analogues was observed.
