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Human Kisspeptin Receptor (KISS1R) ELISA Kit

SKU:
HUDL01616
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q969F8
Sensitivity:
0.126ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
AXOR12,GPR54,KiSS1-Derived Peptide Receptor,G Protein-Coupled Receptor 54,Hypogonadotropin-1,Kisspeptins receptor,Metastin receptor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Kisspeptin Receptor (KISS1R) ELISA Kit

The Human KISS1R (Kisspeptin Receptor) ELISA Kit is specifically designed for the precise quantification of Kisspeptin receptor levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for various research applications.The Kisspeptin receptor, known as KISS1R, is a key protein involved in the regulation of reproductive functions and hormone secretion.

It plays a crucial role in the control of puberty, gonadotropin release, and fertility, making it a valuable biomarker for studying reproductive disorders and potential therapeutic interventions.Overall, the Human KISS1R ELISA Kit provides researchers with a reliable tool to study the role of the Kisspeptin receptor in various physiological and pathological conditions, offering insights into reproductive health and potential treatment strategies.

Product Name:Human Kisspeptin Receptor (KISS1R) ELISA Kit
Product Code:HUDL01616
Size:96 Assays
Target:KISS1R
Synonyms:AXOR12, GPR54, KiSS1-Derived Peptide Receptor, G Protein-Coupled Receptor 54, Hypogonadotropin-1, Kisspeptins receptor, Metastin receptor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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