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Human Kinesin Family, Member 5B (KIF5B) ELISA Kit

SKU:
HUDL01611
Product Type:
ELISA Kit
Sensitivity:
27.8pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
KNS1,UKHC,KINH,Conventional kinesin heavy chain,Ubiquitous kinesin heavy chain,Kinesin-1 heavy chain
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Kinesin Family, Member 5B (KIF5B) ELISA Kit

The Human Kinesin Family Member 5B (KIF5B) ELISA Kit is specifically designed for the accurate detection of KIF5B levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring consistent and reliable results for a variety of research applications.KIF5B is a vital member of the kinesin family, involved in intracellular transport and cell division processes. Dysregulation of this protein has been linked to various diseases, including cancer, neurodegenerative disorders, and developmental abnormalities.

The Human KIF5B ELISA Kit provides an essential tool for studying the role of KIF5B in these conditions and exploring potential therapeutic interventions.With its user-friendly format and robust performance, the Human KIF5B ELISA Kit is a valuable asset for researchers seeking to delve deeper into the functions and implications of KIF5B in human health and disease.

Product Name:Human Kinesin Family, Member 5B (KIF5B) ELISA Kit
Product Code:HUDL01611
Size:96 Assays
Target:KIF5B
Synonyms:KNS1, UKHC, KINH, Conventional kinesin heavy chain, Ubiquitous kinesin heavy chain, Kinesin-1 heavy chain
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9994
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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