The Human Kinesin Family Member 5A (KIF5A) ELISA Kit is specifically designed for the accurate detection of KIF5A levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reliable results for a variety of research applications.Kinesin Family Member 5A (KIF5A) is a motor protein that plays a critical role in intracellular transport and cell division processes. Variations in KIF5A expression have been linked to neurodegenerative disorders, such as amyotrophic lateral sclerosis (ALS) and hereditary spastic paraplegia.
As such, this ELISA kit serves as a valuable tool for studying KIF5A levels in various disease states and exploring potential therapeutic interventions.Overall, the Human Kinesin Family Member 5A (KIF5A) ELISA Kit is an essential resource for researchers seeking to investigate the role of KIF5A in health and disease, ultimately advancing our understanding of cellular dynamics and molecular mechanisms.
Product Name:
Human Kinesin Family, Member 5A (KIF5A) ELISA Kit
Product Code:
HUDL01610
Size:
96 Assays
Target:
KIF5A
Synonyms:
NKHC, MY050, SPG10, Spastic Paraplegia 10(Autosomal Dominant), Kinesin heavy chain neuron-specific 1, Neuronal kinesin heavy chain
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
