The Kidney Injury Molecule 1 (KIM-1) ELISA Kit is purposefully designed for the precise quantitative assessment of human KIM-1 levels in a variety of biological samples. KIM-1, a transmembrane glycoprotein, serves as a biomarker for kidney injury and is upregulated in response to renal damage. Monitoring KIM-1 levels enables the early detection and evaluation of kidney injury, aiding in the diagnosis and prognosis of renal disorders. Accurate measurement of KIM-1 is crucial for monitoring kidney health, assessing renal function, and guiding appropriate clinical interventions.
Our KIM-1 ELISA Kit delivers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research and diagnostic endeavors. Manufactured under stringent quality control standards, this kit guarantees reliable performance and user-friendly protocols, making it an ideal choice for both basic research and clinical applications. Rely on Assay Genie's KIM-1 ELISA Kit for precise and trustworthy quantification of this vital kidney injury biomarker in your studies.
Product Name:
Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit
SKU:
AEES00065
Target:
Human KIM-1 (Kidney Injury Molecule 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KIM-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KIM-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KIM-1. You can calculate the concentration of Human KIM-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-105
94-101
88-96
Average (%)
99
97
91
1:4
Range (%)
94-103
98-105
97-111
Average (%)
98
102
105
1:8
Range (%)
89-97
84-97
97-109
Average (%)
93
92
103
1:16
Range (%)
90-103
100-108
93-104
Average (%)
97
104
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.94
61.85
217.85
23.48
57.68
231.74
Standard deviation
1.4
2.92
8.78
1.24
2.64
12.14
C V (%)
5.61
4.72
4.03
5.28
4.58
5.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human KIM-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KIM-1 in samples. No significant cross-reactivity or interference between Human KIM-1 and analogues was observed.
