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Human Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) ELISA Kit

SKU:
HUDL01614
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P43629
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD158E,HLA-BW4-specific inhibitory NK cell receptor,CD158 antigen-like family member E,Natural killer-associated transcript 3,p70 NK receptor CL-2/CL-11
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) ELISA Kit

The Human Killer Cell Immunoglobulin-Like Receptor 3DL1 (KIR3DL1) ELISA Kit is a cutting-edge tool designed for the accurate quantification of KIR3DL1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results for a variety of research applications.KIR3DL1 is a key immune receptor that plays a critical role in the regulation of natural killer (NK) cell function and immune responses. Dysregulation of KIR3DL1 has been linked to various autoimmune diseases, infectious diseases, and cancer, highlighting its importance as a potential therapeutic target and diagnostic marker.

By utilizing the Human KIR3DL1 ELISA Kit, researchers can gain valuable insights into the role of KIR3DL1 in immune function and disease pathogenesis, paving the way for the development of novel therapeutic strategies and personalized medicine approaches. Trust in this advanced ELISA kit to propel your research forward with its reliable performance and robust results.

Product Name:Human Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) ELISA Kit
Product Code:HUDL01614
Size:96 Assays
Target:KIR3DL1
Synonyms:CD158E, HLA-BW4-specific inhibitory NK cell receptor, CD158 antigen-like family member E, Natural killer-associated transcript 3, p70 NK receptor CL-2/CL-11
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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