null

Human Keratin 5 (KRT5) ELISA Kit

SKU:
HUDL01657
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P13647
Sensitivity:
6.2pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CK5,DDD,EBS2,K5,KRT5A,KRT5-A,Cytokeratin 5,58 kDa cytokeratin,Type-II keratin Kb5,Epidermolysis Bullosa Simplex Dowling-Meara/Kobner/Weber-Cockayne Types
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Keratin 5 (KRT5) ELISA Kit

The Human Keratin 5 (KRT5) ELISA Kit is a highly sensitive and accurate tool for detecting levels of keratin 5 in human samples such as serum, plasma, and cell culture supernatants. This kit offers reliable and consistent results, making it suitable for various research applications.Keratin 5 is a key protein that plays a crucial role in maintaining the structural integrity of epithelial cells, particularly in the skin. Abnormalities in keratin 5 expression have been linked to skin disorders such as epidermolysis bullosa simplex and other genetic conditions that affect the skin's barrier function.

By accurately measuring keratin 5 levels, researchers can gain valuable insights into the mechanisms underlying these skin disorders and potentially identify new treatment targets. The Human Keratin 5 (KRT5) ELISA Kit is a valuable tool for studying the role of keratin 5 in skin biology and exploring potential therapeutic interventions.

Product Name:Human Keratin 5 (KRT5) ELISA Kit
Product Code:HUDL01657
Size:96 Assays
Target:KRT5
Synonyms:CK5, DDD, EBS2, K5, KRT5A, KRT5-A, Cytokeratin 5, 58 kDa cytokeratin, Type-II keratin Kb5, Epidermolysis Bullosa Simplex Dowling-Meara/Kobner/Weber-Cockayne Types
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products