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Human Keratin 1 (KRT1) ELISA Kit (HUDL01651)

SKU:
HUDL01651
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04264
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CK1,EHK1,K1,KRT1A,KRT1-A,Cytokeratin 1,Epidermolytic Hyperkeratosis,Keratin, type II cytoskeletal 1,67 kDa cytokeratin,Type-II keratin Kb1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Keratin 1 (KRT1) ELISA Kit

The Human Keratin 1 (KRT1) ELISA Kit is specifically designed for the accurate and sensitive detection of KRT1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications. Keratin 1 is a key structural protein found in the epidermis, where it provides strength and stability to the skin. Abnormalities in KRT1 expression have been associated with skin disorders such as ichthyosis and epidermolytic hyperkeratosis, making it an important biomarker for studying these conditions.

By using the Human Keratin 1 ELISA Kit, researchers can gain valuable insights into the role of KRT1 in skin health and disease, ultimately leading to a better understanding of skin conditions and potential therapeutic interventions.

Product Name:Human Keratin 1 (KRT1) ELISA Kit
Product Code:HUDL01651
Size:96 Assays
Target:KRT1
Synonyms:CK1, EHK1, K1, KRT1A, KRT1-A, Cytokeratin 1, Epidermolytic Hyperkeratosis, Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Type-II keratin Kb1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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