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Human Kallikrein 3 (KLK3) ELISA Kit

SKU:
HUDL01630
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07288
Sensitivity:
0.7ng/mL
Range:
1.953-125ng/mL
ELISA Type:
Sandwich
Synonyms:
APS,KLK2A1,PSA,HK3,KLKB1,Seminin,Prostate Specific Antigen,Kallikrein-Related Peptidase 3,Semenogelase, g-Seminoprotein,P-30 Antigen,Plasma kallikrein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Kallikrein 3 (KLK3) ELISA Kit

The Human Kallikrein 3 (KLK3) ELISA Kit is a reliable and accurate tool for the detection of KLK3 levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a variety of research applications.KLK3, also known as prostate-specific antigen (PSA), is an important protein involved in prostate function and health. Elevated levels of KLK3 in the blood can indicate prostate cancer or other prostate-related conditions, making it a valuable biomarker for diagnosis and monitoring of such diseases.

By using the Human KLK3 ELISA Kit, researchers can effectively measure KLK3 levels in biological samples, aiding in the study of prostate health, disease progression, and potential therapeutic interventions. Invest in this kit for reliable and accurate detection of KLK3 and advance your research in the field of prostate-related conditions.

Product Name:Human Kallikrein 3 (KLK3) ELISA Kit
Product Code:HUDL01630
Size:96 Assays
Target:KLK3
Synonyms:APS, KLK2A1, PSA, HK3, KLKB1, Seminin, Prostate Specific Antigen, Kallikrein-Related Peptidase 3, Semenogelase, g-Seminoprotein, P-30 Antigen, Plasma kallikrein
Detection Method:Sandwich
Range:1.953-125ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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