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Human Kallikrein 14 (KLK14) ELISA Kit

SKU:
HUDL01627
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9P0G3
Sensitivity:
0.044ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
KLK-L6,Kallikrein-like protein 6
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Kallikrein 14 (KLK14) ELISA Kit

The Human Kallikrein 14 (KLK14) ELISA Kit is a powerful tool for the precise quantification of KLK14 levels in human biological samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.KLK14, a member of the kallikrein-related peptidase family, is involved in various physiological processes such as skin and hair follicle development, inflammation, and cancer progression.

Abnormal expression of KLK14 has been linked to several diseases, making it a valuable biomarker for studying these conditions and potentially developing new therapies.With the Human Kallikrein 14 (KLK14) ELISA Kit, researchers can confidently analyze KLK14 levels in biological samples, advancing their understanding of disease mechanisms and identifying potential therapeutic targets.

Product Name:Human Kallikrein 14 (KLK14) ELISA Kit
Product Code:HUDL01627
Size:96 Assays
Target:KLK14
Synonyms:KLK-L6, Kallikrein-like protein 6
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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