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Human Juxtanodin (JN) ELISA Kit

SKU:
HUDL01591
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8TAM6
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ERMN,Ermin,ERM-Like Protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Human Juxtanodin (JN) ELISA Kit

The Human Juxtanodin (JN) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of juxtanodin levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is ideal for research applications requiring precise quantification of juxtanodin, a protein involved in cell adhesion and regulatory processes.Juxtanodin plays a crucial role in cell-cell interactions and has been implicated in various cellular functions, making it a valuable biomarker for studying diseases such as cancer, cardiovascular diseases, and neurodegenerative disorders.

The reliable and reproducible results obtained with this ELISA kit make it an essential tool for researchers looking to investigate the role of juxtanodin in health and disease.

Product Name:Human Juxtanodin (JN) ELISA Kit
Product Code:HUDL01591
Size:96 Assays
Target:JN
Synonyms:ERMN, Ermin,ERM-Like Protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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