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Human Junctional Adhesion Molecule 1 (JAM1) ELISA Kit

SKU:
HUDL01587
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y624
Sensitivity:
0.133ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD321,F11R,KAT,JAM-A,JAMA,JCAM,PAM1,Coagulation Factor XI Receptor,Platelet F11 receptor,Platelet adhesion molecule 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Junctional Adhesion Molecule 1 (JAM1) ELISA Kit

The Human Junctional Adhesion Molecule-1 (JAM1) ELISA Kit is specifically designed for the accurate and reliable detection of JAM1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications.JAM1 is a key protein involved in cell adhesion and regulation of leukocyte trafficking, playing a crucial role in various physiological and pathological processes such as inflammation, immune response, and cancer metastasis.

By measuring JAM1 levels, researchers can gain valuable insights into these processes and potentially identify new therapeutic targets.Overall, the Human JAM1 ELISA Kit is an essential tool for studying the role of JAM1 in health and disease, providing researchers with the means to advance their understanding of cell adhesion and related pathways.

Product Name:Human Junctional Adhesion Molecule 1 (JAM1) ELISA Kit
Product Code:HUDL01587
Size:96 Assays
Target:JAM1
Synonyms:CD321, F11R, KAT, JAM-A, JAMA, JCAM, PAM1, Coagulation Factor XI Receptor, Platelet F11 receptor, Platelet adhesion molecule 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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