Human ITPK1 Recombinant Protein (RPPB2555)
- SKU:
- RPPB2555
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q13572
- Research Area:
- Enzymes
Description
| Product Name: | Human ITPK1 Recombinant Protein |
| Product Code: | RPPB2555 |
| Size: | 20µg |
| Species: | Human |
| Target: | ITPK1 |
| Synonyms: | Inositol-tetrakisphosphate 1-kinase, Inositol 1,3,4-trisphosphate 5/6-kinase, Inositol-triphosphate 5/6-kinase, Ins(1,3,4)P(3) 5/6-kinase, ITPK1, ITRPK1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | ITPK1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 10% glycerol and 1mM EDTA. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMQTFLK GKRVGYWLSE KKIKKLNFQA FAELCRKRGM EVVQLNLSRP IEEQGPLDVI IHKLTDVILE ADQNDSQSLE LVHRFQEYID AHPETIVLDP LPAIRTLLDR SKSYELIRKI EAYMEDDRIC SPPFMELTSL CGDDTMRLLE KNGLTFPFIC KTRVAHGTNS HEMAIVFNQE GLNAIQPPCV VQNFINHNAV LYKVFVVGES YTVVQRPSLK NFSAGTSDRE SIFFNSHNVS KPESSSVLTE LDKIEGVFER PSDEVIRELS RALRQALGVS LFGIDIIINN QTGQHAVIDI NAFPGYEGVS EFFTDLLNHI ATVLQGQSTA MAATGDVALL RHSKLLAEPA GGLVGERTCS ASPGCCGSMM GQDAPWKAEA DAGGTAKLPH QRLGCNAGVS PSFQQHCVAS LATKASSQ |
Inositol-tetrakisphosphate 1-kinase (ITPK1) is a 414 amino acid monomer which is a member of the ITPK1 family and exists as 2 alternatively spliced isoforms. ITPK1 highest levels are found in the brain, followed by the heart, skeletal muscle, kidney, pancreas, liver, placenta and lung. ITPK1 is comprised of one ATP-grasp domain and has been shown to phosphorylate various inositol polyphosphates and modify TNFa induced apoptosis.
ITPK1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 438 amino acids (1-414 a.a) and having a molecular mass of 48.1kDa.ITPK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | ITPK1: Kinase that can phosphorylate various inositol polyphosphate such as Ins(3,4,5,6)P4 or Ins(1,3,4)P3. Phosphorylates Ins(3,4,5,6)P4 at position 1 to form Ins(1,3,4,5,6)P5. This reaction is thought to have regulatory importance, since Ins(3,4,5,6)P4 is an inhibitor of plasma membrane Ca(2+)-activated Cl(-) channels, while Ins(1,3,4,5,6)P5 is not. Also phosphorylates Ins(1,3,4)P3 on O-5 and O-6 to form Ins(1,3,4,6)P4, an essential molecule in the hexakisphosphate (InsP6) pathway. Also acts as an inositol polyphosphate phosphatase that dephosphorylate Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 to Ins(1,3,4)P3, and Ins(1,3,4,5,6)P5 to Ins(3,4,5,6)P4. May also act as an isomerase that interconverts the inositol tetrakisphosphate isomers Ins(1,3,4,5)P4 and Ins(1,3,4,6)P4 in the presence of ADP and magnesium. Probably acts as the rate-limiting enzyme of the InsP6 pathway. Modifies TNF-alpha-induced apoptosis by interfering with the activation of TNFRSF1A-associated death domain. Belongs to the ITPK1 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Carbohydrate Metabolism - inositol phosphate; Kinase, lipid; EC 2.7.1.134; EC 2.7.1.159 Chromosomal Location of Human Ortholog: 14q31 Cellular Component: cytosol Molecular Function:catalytic activity; inositol tetrakisphosphate 1-kinase activity Biological Process: blood coagulation; inositol phosphate metabolic process; signal transduction |
| NCBI Summary: | This gene encodes an enzyme that belongs to the inositol 1,3,4-trisphosphate 5/6-kinase family. This enzyme regulates the synthesis of inositol tetraphosphate, and downstream products, inositol pentakisphosphate and inositol hexakisphosphate. Inositol metabolism plays a role in the development of the neural tube. Disruptions in this gene are thought to be associated with neural tube defects. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Jul 2016] |
| UniProt Code: | Q13572 |
| NCBI GenInfo Identifier: | 83288249 |
| NCBI Gene ID: | 3705 |
| NCBI Accession: | Q13572.2 |
| UniProt Secondary Accession: | Q13572,Q9BTL6, Q9H2E7, |
| UniProt Related Accession: | Q13572 |
| Molecular Weight: | 35,632 Da |
| NCBI Full Name: | Inositol-tetrakisphosphate 1-kinase |
| NCBI Synonym Full Names: | inositol-tetrakisphosphate 1-kinase |
| NCBI Official Symbol: | ITPK1�� |
| NCBI Official Synonym Symbols: | ITRPK1�� |
| NCBI Protein Information: | inositol-tetrakisphosphate 1-kinase |
| UniProt Protein Name: | Inositol-tetrakisphosphate 1-kinase |
| UniProt Synonym Protein Names: | Inositol 1,3,4-trisphosphate 5/6-kinase (EC:2.7.1.159); Inositol-triphosphate 5/6-kinase; Ins(1,3,4)P(3) 5/6-kinase |
| Protein Family: | Inositol-tetrakisphosphate 1-kinase |
| UniProt Gene Name: | ITPK1�� |
| UniProt Entry Name: | ITPK1_HUMAN |
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