null

Human ITCH(E3 ubiquitin-protein ligase Itchy homolog) Elisa Kit

SKU:
AEFI00262
Product Type:
ELISA Kit
Size:
96 Assays
Sensitivity:
18.75pg/ml
Range:
31.25-2000pg/ml
ELISA Type:
Sandwich
Reactivity:
Human
€599
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Human ITCH (E3 ubiquitin-protein ligase Itchy homolog) ELISA Kit

The Human Itch E3 Ubiquitin Protein Ligase (ITCH) ELISA Kit is a reliable and accurate tool for detecting levels of the ITCH protein in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing consistent and trustworthy results for various research applications.ITCH is a critical enzyme involved in the regulation of protein degradation and cell signaling pathways. Its dysregulation has been linked to various diseases, including cancer, autoimmune disorders, and inflammatory conditions.

Therefore, the ITCH protein serves as a valuable biomarker for studying these diseases and developing potential therapeutic interventions.With the Human Itch E3 Ubiquitin Protein Ligase (ITCH) ELISA Kit, researchers can efficiently measure ITCH protein levels in human samples, advancing their understanding of the molecular mechanisms underlying disease pathology and paving the way for innovative treatment approaches.

Product Name:Human ITCH(E3 ubiquitin-protein ligase Itchy homolog) Elisa Kit
Product Code:AEFI00262
Size:96T
Alias:E3 ubiquitin-protein ligase Itchy homolog, Itch, Atrophin-1-interacting protein 4, AIP4, HECT-type E3 ubiquitin transferase Itchy homolog, NFE2-associated polypeptide 1, NAPP1, ITCH
Detection method:Sandwich ELISA, Double Antibody
Application:ITCH ELISA Kit allows for the in vitro quantitative determination of ITCH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Sensitivity:< 18.75pg/ml
Range:31.25-2000pg/ml
Storage:2-8°C for 6 months
Note:For Research Use Only
Recovery: Matrices listed below were spiked with certain level of ITCH and the recovery rates were calculated by comparing the measured value to the expected amount of ITCH in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)96-10399
EDTA plasma(n=5)96-10499
heparin plasma(n=5)95-103100
Linearity: The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ITCH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)90-105%91-105%91-105%
EDTA plasma(n=5)89-100%89-100%90-98%
heparin plasma(n=5)90-99%94-99%91-100%
CV(%): Intra-Assay: CV<8%
Inter-Assay: CV<10%

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
Serum If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

Related Products