Human Isocitrate Dehydrogenase 1, Soluble (IDH1) ELISA Kit
The Human Isocitrate Dehydrogenase 1 (IDH1) ELISA Kit is specifically designed for the accurate measurement of soluble IDH1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results that are essential for various research applications.IDH1 is a key enzyme involved in cellular metabolism and is commonly mutated in various types of cancers, making it a valuable biomarker for cancer research and diagnostics. This ELISA kit provides researchers with a powerful tool to quantitatively assess IDH1 levels, aiding in the study of cancer progression and potential therapeutic interventions.
Overall, the Human Isocitrate Dehydrogenase 1 (IDH1) ELISA Kit is a valuable resource for scientists and clinicians working in the field of cancer research and personalized medicine. With its high performance and ease of use, this kit is essential for advancing our understanding of IDH1 biology and its implications in cancer development and treatment strategies.
Product Name:
Human Isocitrate Dehydrogenase 1, Soluble (IDH1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
