Human Iron Responsive Element Binding Protein 2 (IREB2) ELISA Kit
The Human Iron-Responsive Element Binding Protein 2 (IREB2) ELISA Kit is a powerful tool for accurately measuring the levels of IREB2 in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable resource for a variety of research applications.IREB2 is a key protein involved in iron metabolism, regulating the expression of genes responsible for iron uptake and storage. Dysregulation of IREB2 has been linked to disorders such as hereditary hemochromatosis and neurodegenerative diseases.
Studying IREB2 levels can provide valuable insights into the mechanisms underlying these conditions and aid in the development of new therapeutic strategies.Whether investigating the role of IREB2 in iron homeostasis or exploring its potential as a biomarker for certain diseases, the Human IREB2 ELISA Kit offers a reliable and accurate solution for researchers in the field. Trust in this kit to deliver precise and meaningful data to advance your research efforts.
Product Name:
Human Iron Responsive Element Binding Protein 2 (IREB2) ELISA Kit
Product Code:
HUDL01549
Size:
96 Assays
Target:
IREB2
Synonyms:
IRP2, Iron regulatory protein 2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
