Human Irisin ELISA Kit (HUFI03190)
- SKU:
- HUFI03190
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q8NAU1
- Sensitivity:
- 0.938ng/ml
- Range:
- 1.563-100ng/ml
- ELISA Type:
- Sandwich
- Synonyms:
- Irisin
- Reactivity:
- Human
Description
Human Irisin ELISA Kit
Irisin, a fascinating hormone, has emerged as a key player in the field of human physiology and exercise-related research. Irisin is a small protein hormone that is released by our muscles during physical activity and acts as a molecular messenger, orchestrating a range of beneficial effects throughout the body. Irisin has garnered significant attention for its potential role in regulating metabolism, promoting fat browning, and improving overall health. The Assay Genie Human Irisin ELISA Kit is a highly sensitive assay for the quantitative measurement of Irisin in serum, plasma, cell culture supernatant and tissue samples.
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Human Irisin ELISA Kit |
| Product Code: | HUFI03190 |
| Size: | 96 Assays |
| Alias: | Irisin |
| Detection Method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human Irisin concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.938ng/ml |
| Range: | 1.563-100ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
Additional Information
| Recovery | Matrices listed below were spiked with certain level of Human Irisin and the recovery rates were calculated by comparing the measured value to the expected amount of Human Irisin in samples. Enquire for more information. |
| Linearity | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human Irisin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Enquire for more information. |
| CV(%) | Intra-Assay: CV<8% |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/ -20°C |
| Sample/Standard Dlution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody (Concentrated) | 120ul | 4°C (Protection from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate (SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protection from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protein Information
Protocol
*Note: Protocols are specific to each batch/lot. For the exact instructions please follow the protocol included in your kit.
Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.
| Step | Procedure |
| 1. | Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells! |
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