Human IRF5 (Interferon Regulatory Factor 5) CLIA Kit
The Human IRF5 (Interferon Regulatory Factor 5) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of IRF5 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for various research applications.IRF5 is a key transcription factor that plays a critical role in the regulation of immune responses and inflammation. Dysregulation of IRF5 has been implicated in various autoimmune diseases, including lupus, rheumatoid arthritis, and inflammatory bowel disease.
Therefore, measuring IRF5 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted therapies.With its high precision and ease of use, the Human IRF5 CLIA Kit from Assay Genie is a valuable resource for researchers studying the role of IRF5 in immune disorders and seeking to identify novel therapeutic targets.
Product Name:
Human IRF5 (Interferon Regulatory Factor 5) CLIA Kit
SKU:
HUES00875
Target:
Human IRF5 (Interferon Regulatory Factor 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IRF5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IRF5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IRF5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IRF5. You can calculate the concentration of Human IRF5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
89-104
98-111
Average (%)
93
96
104
1:4
Range (%)
84-98
104-120
102-117
Average (%)
91
110
108
1:8
Range (%)
96-112
87-97
92-103
Average (%)
103
92
98
1:16
Range (%)
103-120
91-106
93-110
Average (%)
110
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
94-105
100
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.57
177.11
685.58
99.81
177.06
668.88
Standard deviation
10.12
20.99
43.33
12.89
18.63
40.13
C V (%)
10.7
11.85
6.32
12.91
10.52
6.0
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IRF5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IRF5 in samples. No significant cross-reactivity or interference between Human IRF5 and analogues was observed.
