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Human Interleukin 8 Receptor Alpha (IL-8Ra) ELISA Kit

SKU:
HUDL01527
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P25024
Sensitivity:
0.072ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD181,CDw128a,CXCR1,CKR1,CMKAR1,IL8R1,IL8RBA,IL8-RA,Chemokine C-X-C motif Receptor 1,High Affinity Interleukin-8 Receptor A
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 8 Receptor Alpha (IL-8Ra) ELISA Kit

The Human Interleukin-8 Receptor Alpha (IL8RA) ELISA Kit is a powerful tool for the precise measurement of IL8RA levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.IL8RA is a key receptor involved in the immune response, specifically in the recruitment of neutrophils to sites of inflammation. Dysregulation of IL8RA has been linked to inflammatory diseases, autoimmune disorders, and cancer.

Therefore, the IL8RA ELISA Kit is essential for investigating the role of this receptor in disease pathogenesis and for developing targeted therapies.With its advanced technology and reliability, the Human IL8RA ELISA Kit from Assay Genie is a valuable tool for researchers seeking to delve deeper into the complex mechanisms of inflammation and immune response in various health conditions.

Product Name:Human Interleukin 8 Receptor Alpha (IL-8Ra) ELISA Kit
Product Code:HUDL01527
Size:96 Assays
Target:IL8Ra
Synonyms:CD181, CDw128a, CXCR1, CKR1, CMKAR1, IL8R1, IL8RBA, IL8-RA, Chemokine C-X-C motif Receptor 1, High Affinity Interleukin-8 Receptor A
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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