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Human Interleukin 8 (IL-8) ELISA Kit

SKU:
HUDL01526
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10145
Sensitivity:
3.6pg/mL
Range:
7.81-500pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL8,AMCF-I,GCP1,K60,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP1,SCYB8,TSG1,B-ENAP,Neutrophil-Activating Protein 1,Granulocyte Chemotactic Protein 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 8 (IL-8) ELISA Kit

The Human Interleukin-8 (IL-8) ELISA Kit is specifically designed for the precise quantification of IL-8 levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for various research applications.IL-8 is a key chemokine involved in inflammation and immune response, playing a crucial role in recruiting immune cells to the site of infection or injury.

Elevated levels of IL-8 have been associated with various inflammatory diseases, such as asthma, rheumatoid arthritis, and inflammatory bowel disease, making it a critical biomarker for studying these conditions and developing potential therapies.Overall, the Human Interleukin-8 (IL-8) ELISA Kit provides researchers with a reliable and efficient method for analyzing IL-8 levels, furthering our understanding of inflammatory processes and potential treatment strategies.

Product Name:Human Interleukin 8 (IL-8) ELISA Kit
Product Code:HUDL01526
Size:96 Assays
Target:IL8
Synonyms:CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1
Detection Method:Sandwich
Range:7.81-500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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