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Human Interleukin 20 (IL-20) ELISA Kit

SKU:
HUDL01493
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9NYY1
Sensitivity:
5.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
IL10D,IL10-D,ZCYTO10,Cytokine Zcyto10
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 20 (IL-20) ELISA Kit

The Human Interleukin-20 (IL-20) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of IL-20 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.IL-20 is a cytokine that plays a key role in inflammatory responses and immune regulation. It is implicated in various inflammatory diseases, autoimmune disorders, and cancer.

By measuring IL-20 levels, researchers can better understand the role of this cytokine in disease pathology and potentially develop targeted therapies.Overall, the Human Interleukin-20 (IL-20) ELISA Kit is a valuable tool for researchers studying cytokine-mediated diseases and looking to uncover new insights into the role of IL-20 in human health and disease.

Product Name:Human Interleukin 20 (IL-20) ELISA Kit
Product Code:HUDL01493
Size:96 Assays
Target:IL20
Synonyms:IL10D, IL10-D, ZCYTO10, Cytokine Zcyto10
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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