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Human Interleukin 2 Receptor Gamma (IL-2Rg) ELISA Kit

SKU:
HUDL01510
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P31785
Sensitivity:
0.067ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD132,IL2R-g,IL2-RG,CD132,IMD4,p64,SCIDX,SCIDX1,Cytokine Receptor Common Gamma Chain,Severe Combined Immunodeficiency
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 2 Receptor Gamma (IL-2Rg) ELISA Kit

The Human Interleukin-2 Receptor Gamma (IL2RG) ELISA Kit is a highly reliable and sensitive assay designed for the accurate quantification of IL2RG levels in human samples including serum, plasma, and cell culture supernatants. This kit provides researchers with a powerful tool for studying the role of IL2RG in various biological processes.IL2RG is a key component of the interleukin-2 receptor complex, playing a critical role in immune system function and regulation.

Dysregulation of IL2RG has been associated with various immune disorders and diseases, making it a valuable biomarker for research and clinical applications.With its high sensitivity and specificity, the IL2RG ELISA Kit offers precise and reproducible results, enabling researchers to confidently assess IL2RG levels in their samples. This kit is a valuable resource for investigating the role of IL2RG in immune responses, inflammation, and disease pathogenesis.

Product Name:Human Interleukin 2 Receptor Gamma (IL-2Rg) ELISA Kit
Product Code:HUDL01510
Size:96 Assays
Target:IL2Rg
Synonyms:CD132, IL2R-g, IL2-RG, CD132, IMD4, p64, SCIDX, SCIDX1, Cytokine Receptor Common Gamma Chain, Severe Combined Immunodeficiency
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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