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Human Interleukin 2 Receptor Beta (IL-2Rb) ELISA Kit

SKU:
HUDL01509
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14784
Sensitivity:
28pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD122,IL2R-b,IL2-RB,P70-75,High affinity IL-2 receptor subunit beta,Interleukin-2 receptor subunit beta,IL-2 receptor subunit beta
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 2 Receptor Beta (IL-2Rb) ELISA Kit

The Human Interleukin-2 Receptor Beta (IL2RB) ELISA Kit is specifically designed for the accurate detection of interleukin-2 receptor beta levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Interleukin-2 receptor beta is a critical component in the immune response, playing a key role in T-cell activation and proliferation. Dysregulation of IL2RB levels has been associated with autoimmune diseases, inflammatory disorders, and immune deficiencies, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.

With its advanced technology and user-friendly design, the Human IL2RB ELISA Kit provides researchers with a powerful tool to explore the role of interleukin-2 receptor beta in health and disease. Trust in Assay Genie to deliver accurate and dependable results for your research needs.

Product Name:Human Interleukin 2 Receptor Beta (IL-2Rb) ELISA Kit
Product Code:HUDL01509
Size:96 Assays
Target:IL2Rb
Synonyms:CD122, IL2R-b, IL2-RB, P70-75, High affinity IL-2 receptor subunit beta, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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