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Human Interleukin 2 Receptor Alpha (IL-2Ra) ELISA Kit

SKU:
HUDL01508
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01589
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD25,IL2R-a,IL2-RA,IDDM10,IL2R,TCGFR,p55,TAC antigen
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 2 Receptor Alpha (IL-2Ra) ELISA Kit

The Human Interleukin-2 Receptor Alpha (IL2RA) ELISA Kit is a powerful tool for accurately measuring IL2RA levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research applications.IL2RA is a key receptor involved in the immune response, particularly in T cell activation and regulation. Changes in IL2RA levels have been linked to autoimmune diseases, organ transplant rejection, and immune system dysregulation.

As such, this ELISA kit is essential for studying these conditions and developing potential therapeutic interventions.Overall, the Human IL2RA ELISA Kit from Assay Genie provides researchers with a reliable and efficient method for quantifying IL2RA levels, ultimately advancing our understanding of immune system function and associated diseases.

Product Name:Human Interleukin 2 Receptor Alpha (IL-2Ra) ELISA Kit
Product Code:HUDL01508
Size:96 Assays
Target:IL2Ra
Synonyms:CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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