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Human Interleukin 18 Binding Protein (IL-18BP) ELISA Kit

SKU:
HUDL01477
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O95998
Sensitivity:
0.51ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
IL18BPa,MC51L-53L-54L Homolog Gene Product,Tadekinig-alfa
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Human Interleukin 18 Binding Protein (IL-18BP) ELISA Kit

The Human Interleukin-18 Binding Protein (IL18BP) ELISA Kit is a powerful tool for the accurate quantification of IL18BP levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for various research applications.IL18BP is a key regulatory protein that binds to and neutralizes interleukin-18, a pro-inflammatory cytokine involved in immune responses and inflammatory processes. By measuring IL18BP levels, researchers can gain valuable insights into the modulation of interleukin-18 activity and its impact on various disease states such as autoimmune disorders, inflammatory conditions, and infectious diseases.

With its high performance and versatility, the Human IL18BP ELISA Kit is an indispensable tool for studying the role of interleukin-18 binding protein in health and disease, providing valuable information for the development of targeted therapies and diagnostic strategies.

Product Name:Human Interleukin 18 Binding Protein (IL-18BP) ELISA Kit
Product Code:HUDL01477
Size:96 Assays
Target:IL18BP
Synonyms:IL18BPa, MC51L-53L-54L Homolog Gene Product, Tadekinig-alfa
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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