Human Interleukin 17B (IL-17B) ELISA Kit
- SKU:
- HUDL01471
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q9UHF5
- Sensitivity:
- 5.8pg/mL
- Range:
- 15.625-1000pg/mL
- ELISA Type:
- Sandwich
- Synonyms:
- IL-20,Cytokine Zcyto7,Neuronal interleukin-17-related factor
- Reactivity:
- Human
- Sample Type:
- Serum, Plasma And Other Biological Fluids
Frequently bought together:
Description
system_update_altDatasheet
Human Interleukin 17B (IL-17B) ELISA Kit
The Human Interleukin 17B (IL17B) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of IL17B levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a variety of research applications.Interleukin 17B is a cytokine that plays a crucial role in inflammation and immune response regulation. Abnormal IL17B levels have been associated with various inflammatory conditions and diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
The Human IL17B ELISA Kit from Assay Genie provides researchers with a powerful tool for studying the role of IL17B in health and disease, offering a comprehensive solution for accurate and precise measurement of IL17B levels in biological samples.
| Product Name: | Human Interleukin 17B (IL-17B) ELISA Kit |
| Product Code: | HUDL01471 |
| Size: | 96 Assays |
| Target: | IL17B |
| Synonyms: | IL-20, Cytokine Zcyto7, Neuronal interleukin-17-related factor |
| Detection Method: | Sandwich |
| Range: | 15.625-1000pg/mL |
| Shelf Life: | 12 months |
| Note: | For research use only |
| Recovery: | Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples. | ||||||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||||||
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| Intra-assay Precision: | Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively. | ||||||||||||||||||||
| Inter-assay Precision: | Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate. | ||||||||||||||||||||
| Stability: | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Step | Protocol |
| 1. | Prepare all reagents, samples and standards |
| 2. | Add 100µL standard or sample to each well. Incubate 2 hours at 37°C |
| 3. | Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C |
| 4. | Aspirate and wash 3 times |
| 5. | Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C |
| 6. | Aspirate and wash 5 times |
| 7. | Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C |
| 8. | Add 50µL Stop Solution. Read at 450nm immediately. |
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