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Human Interleukin 12B (IL-12B) ELISA Kit

SKU:
HUDL01464
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29460
Sensitivity:
11.84pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
p40,IL12-B,CLMF2,NKSF2,Natural Killer Cell Stimulatory Factor 2,Cytotoxic Lymphocyte Maturation Factor 2,Interleukin 23, P40 Subunit
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Human Interleukin 12B (IL-12B) ELISA Kit

The Human Interleukin-12B (IL12B) ELISA Kit is specifically designed for the accurate detection of Interleukin-12B levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring reliable and reproducible results for a variety of research applications.Interleukin-12B is a key cytokine involved in the regulation of immune responses, playing a critical role in the development of Th1 cells and the production of interferon gamma.

Dysregulation of Interleukin-12B has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.With its advanced technology and ease of use, the Human Interleukin-12B (IL12B) ELISA Kit is an essential tool for researchers looking to study the role of Interleukin-12B in immunology and disease pathogenesis.

Product Name:Human Interleukin 12B (IL-12B) ELISA Kit
Product Code:HUDL01464
Size:96 Assays
Target:IL12B
Synonyms:p40, IL12-B, CLMF2, NKSF2, Natural Killer Cell Stimulatory Factor 2, Cytotoxic Lymphocyte Maturation Factor 2, Interleukin 23, P40 Subunit
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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