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Human Interleukin 12A (IL-12A) ELISA Kit

SKU:
HUDL01463
Product Type:
ELISA Kit
Sensitivity:
11.9pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
p35,IL12-A,CLMF1,NFSK,NKSF1,Natural Killer Cell Stimulatory Factor 1,Cytotoxic Lymphocyte Maturation Factor 1,NF Cell Stimulatory Factor Chain 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

system_update_altDatasheet

Human Interleukin 12A (IL-12A) ELISA Kit

The Human Interleukin-12A (IL-12A) ELISA Kit is a powerful tool for the quantitative measurement of interleukin-12A levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it perfect for a variety of research applications.Interleukin-12A is a key cytokine involved in the regulation of immune responses, playing a crucial role in inflammatory and immune-mediated diseases.

Its dysregulation has been linked to conditions such as autoimmune disorders, infections, and cancer, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.With the Human Interleukin-12A (IL-12A) ELISA Kit, researchers can gain valuable insights into the role of interleukin-12A in health and disease, paving the way for innovative treatments and improved patient outcomes.

Product Name:Human Interleukin 12A (IL-12A) ELISA Kit
Product Code:HUDL01463
Size:96 Assays
Synonyms:p35, IL12-A, CLMF1, NFSK, NKSF1, Natural Killer Cell Stimulatory Factor 1, Cytotoxic Lymphocyte Maturation Factor 1, NF Cell Stimulatory Factor Chain 1
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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