null

Human Interferon Regulatory Factor 9 (IRF9) ELISA Kit

SKU:
HUDL01555
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q00978
Sensitivity:
0.042ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ISGF3G,ISGF3,p48,Interferon-Stimulated Transcription Factor 3,Gamma 48kDa,IFN-alpha-responsive transcription factor subunit,Interferon-stimulated gene factor 3 gamma
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Interferon Regulatory Factor 9 (IRF9) ELISA Kit

The Human Interferon Regulatory Factor 9 (IRF9) ELISA Kit is a powerful tool for the precise measurement of IRF9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.IRF9 is a key transcription factor that plays a critical role in regulating interferon signaling and immune responses. Dysregulation of IRF9 has been implicated in various autoimmune diseases, viral infections, and inflammatory disorders, making it an important target for studying disease mechanisms and potential therapeutic interventions.

With its advanced technology and user-friendly protocol, the Human IRF9 ELISA Kit is an essential tool for researchers seeking to unravel the complex role of IRF9 in health and disease. Trust in this kit to deliver reliable data and insights that can drive groundbreaking discoveries in the fields of immunology, virology, and beyond.

Product Name:Human Interferon Regulatory Factor 9 (IRF9) ELISA Kit
Product Code:HUDL01555
Size:96 Assays
Target:IRF9
Synonyms:ISGF3G, ISGF3, p48, Interferon-Stimulated Transcription Factor 3,Gamma 48kDa, IFN-alpha-responsive transcription factor subunit, Interferon-stimulated gene factor 3 gamma
Detection Method:Sandwich
Range:0.156-10 ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products