null

Human Interferon Alpha 4 (IFNa4) ELISA Kit

SKU:
HUDL01437
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05014
Sensitivity:
5.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
INFA4,Interferon Alpha-4B,Interferon Alpha-76,Interferon Alpha-M1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Interferon Alpha 4 (IFNa4) ELISA Kit

The Human Interferon Alpha-4 (IFNA4) ELISA Kit is specially designed for the precise and reliable quantification of IFNA4 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an excellent tool for various research applications.IFNA4 is a crucial type I interferon that plays a vital role in the immune response against viral infections and cancer. By measuring IFNA4 levels, researchers can gain valuable insights into the immune system's response to pathogens and potentially develop new therapies for infectious diseases and cancer.

Overall, the Human IFNA4 ELISA Kit is a valuable asset for researchers looking to study the role of IFNA4 in immune responses and disease pathogenesis. Its high-performance characteristics ensure precise and consistent results, making it a reliable choice for your research needs.

Product Name:Human Interferon Alpha 4 (IFNa4) ELISA Kit
Product Code:HUDL01437
Size:96 Assays
Target:IFNa4
Synonyms:INFA4, Interferon Alpha-4B, Interferon Alpha-76, Interferon Alpha-M1
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products