Human Inter Alpha-Globulin Inhibitor H3 (ITIH3) ELISA Kit
The Human ITIH3 (Inter-alpha-Trypsin Inhibitor Heavy Chain 3) ELISA Kit is specifically designed for the precise measurement of ITIH3 levels in human serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit guarantees accurate and consistent results, making it an indispensable tool for various research purposes.ITIH3 is a key protein that acts as an inhibitor for certain enzymes and plays a crucial role in regulating inflammatory responses in the body. Dysregulation of ITIH3 expression has been linked to inflammatory disorders, cancer, and various other diseases, highlighting its significance as a valuable biomarker for investigating these conditions and exploring potential therapeutic interventions.
With the Human ITIH3 ELISA Kit, researchers can uncover new insights into the role of ITIH3 in disease pathogenesis and progression, ultimately paving the way for innovative diagnostic and treatment strategies. Trust in this kit to deliver reliable data and drive groundbreaking discoveries in the field of biomedical research.
Product Name:
Human Inter Alpha-Globulin Inhibitor H3 (ITIH3) ELISA Kit
Product Code:
HUDL01577
Size:
96 Assays
Target:
ITIH3
Synonyms:
H3P, SHAP, Pre-Alpha(Globulin)Inhibitor,H3 Polypeptide, Serum-derived hyaluronan-associated protein
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
