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Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) ELISA Kit

SKU:
HUDL01576
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19823
Sensitivity:
35pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
H2P,SHAP,IGHEP2,Serum-derived hyaluronan-associated protein,Inter-alpha-trypsin inhibitor complex component II
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) ELISA Kit

The Human Inter Alpha Globulin Inhibitor H2 (ITIH2) ELISA Kit is a powerful tool for accurately measuring levels of ITIH2 in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it an invaluable asset for a wide range of research studies.ITIH2 is a critical protein that plays a key role in various biological processes, including inflammation and immune response regulation.

It is implicated in different diseases, such as cancer and autoimmune disorders, making it a key biomarker for understanding and potentially treating these conditions.By using the Human Inter Alpha Globulin Inhibitor H2 (ITIH2) ELISA Kit, researchers can gain valuable insights into the role of ITIH2 in disease pathogenesis and progression, paving the way for the development of novel diagnostic and therapeutic strategies.

Product Name:Human Inter Alpha-Globulin Inhibitor H2 (ITIH2) ELISA Kit
Product Code:HUDL01576
Size:96 Assays
Target:ITIH2
Synonyms:H2P, SHAP, IGHEP2, Serum-derived hyaluronan-associated protein, Inter-alpha-trypsin inhibitor complex component II
Detection Method:Sandwich
Range:78.125-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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