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Human Integrin Beta 1 (ITGb1) ELISA Kit

SKU:
HUDL01570
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05556
Sensitivity:
0.27ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CD29,MSK12,ITG-B1,FNRB,GPIIA,MDF2,MSK12,VLAB,Fibronectin Receptor Beta Polypeptide,Antigen CD29 Includes MDF2,Glycoprotein IIa,VLA-4 subunit beta
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Integrin Beta 1 (ITGb1) ELISA Kit

The Human Integrin Beta-1 (ITGB1) ELISA Kit is specifically designed for the precise measurement of integrin beta-1 levels in human samples, including serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Integrin beta-1 is a key protein involved in cell adhesion and migration, playing a crucial role in processes like wound healing, immune response, and cancer metastasis.

Dysregulation of integrin beta-1 has been implicated in various diseases, making it a valuable biomarker for studying disease mechanisms and developing therapeutic interventions.With its high performance and reliability, the Human Integrin Beta-1 (ITGB1) ELISA Kit is an essential tool for researchers investigating integrin beta-1's role in health and disease.

Product Name:Human Integrin Beta 1 (ITGb1) ELISA Kit
Product Code:HUDL01570
Size:96 Assays
Target:ITGb1
Synonyms:CD29, MSK12, ITG-B1, FNRB, GPIIA, MDF2, MSK12, VLAB, Fibronectin Receptor Beta Polypeptide, Antigen CD29 Includes MDF2, Glycoprotein IIa, VLA-4 subunit beta
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-9991
EDTA plasma (n=5)80-9989
Heparin plasma (n=5)82-9990
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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