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Human Integrin Alpha M (ITGaM) ELISA Kit

SKU:
HUDL01568
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11215
Sensitivity:
0.125ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD11b,ITG-AM,CR3A,MAC-1,MAC1A,MO1A,Complement Component 3 Receptor 3 Subunit,Cell surface glycoprotein MAC-1 subunit alpha,Neutrophil adherence receptor
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Integrin Alpha M (ITGaM) ELISA Kit

The Human Integrin Alpha M (ITGAM) ELISA Kit is a powerful tool for measuring integrin alpha M levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit has been developed with high sensitivity and specificity to deliver accurate and reproducible results, making it ideal for various research applications.Integrin alpha M is a cell surface protein that plays a crucial role in mediating cell adhesion and migration in immune responses. It is involved in inflammatory processes, immune cell recruitment, and phagocytosis, making it a valuable biomarker for studying immune system disorders, autoimmune diseases, and inflammation-related conditions.

By accurately measuring integrin alpha M levels, researchers can gain insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets. The Human Integrin Alpha M (ITGAM) ELISA Kit provides a reliable and efficient solution for studying integrin alpha M and its implications in human health and disease.

Product Name:Human Integrin Alpha M (ITGaM) ELISA Kit
Product Code:HUDL01568
Size:96 Assays
Target:ITGaM
Synonyms:CD11b, ITG-AM, CR3A, MAC-1, MAC1A, MO1A, Complement Component 3 Receptor 3 Subunit, Cell surface glycoprotein MAC-1 subunit alpha, Neutrophil adherence receptor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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