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Human Integrin Alpha 2B (ITGa2B) ELISA Kit

SKU:
HUDL01564
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08514
Sensitivity:
0.052ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD41,CD41B,ITG-A2B,GTA,GP2B,GPIIb,HPA3,Platelet Glycoprotein IIb Of IIb/IIIa Complex,Antigen CD41
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Integrin Alpha 2B (ITGa2B) ELISA Kit

The Human Integrin Alpha 2b (ITGA2B) ELISA Kit is specifically designed for the accurate and sensitive detection of ITGA2B levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.ITGA2B is a crucial integrin involved in platelet aggregation and clot formation, playing a vital role in processes such as hemostasis and thrombosis.

Dysregulation of ITGA2B has been linked to various platelet disorders and cardiovascular diseases, making it an important biomarker for studying these conditions and developing potential interventions.Overall, the Human Integrin Alpha 2b (ITGA2B) ELISA Kit provides researchers with a powerful tool for investigating the role of ITGA2B in health and disease, offering reliable and accurate measurement of ITGA2B levels in human samples.

Product Name:Human Integrin Alpha 2B (ITGa2B) ELISA Kit
Product Code:HUDL01564
Size:96 Assays
Target:ITGa2B
Synonyms:CD41, CD41B, ITG-A2B, GTA, GP2B, GPIIb, HPA3, Platelet Glycoprotein IIb Of IIb/IIIa Complex, Antigen CD41
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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