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Human Integrin Alpha 2 (ITGa2) ELISA Kit

SKU:
HUDL01565
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P17301
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD49b,BR,GPIa,VLA-2,VLAA2,Platelet membrane glycoprotein Ia,Alpha 2 Subunit Of VLA-2 Receptor,Very Late Activation Proteins 2 Receptor,Collagen receptor,VLA-2 alpha
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Human Integrin Alpha 2 (ITGa2) ELISA Kit

The Human Integrin Alpha-2 (ITGA2) ELISA Kit is specifically designed to accurately measure ITGA2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures dependable and repeatable results, making it an excellent choice for various research applications.ITGA2 is an important cell adhesion molecule that plays a key role in cell migration, proliferation, and differentiation. Dysregulation of ITGA2 has been linked to various diseases, including cancer, fibrosis, and inflammatory disorders, making it a valuable biomarker for studying these conditions and potentially developing targeted treatments.

By using the Human ITGA2 ELISA Kit, researchers can accurately quantify ITGA2 levels in biological samples, providing valuable insights into its role in disease pathogenesis and potential therapeutic interventions. Trust in the reliability and precision of this kit to advance your research in the field of integrin biology and related diseases.

Product Name:Human Integrin Alpha 2 (ITGa2) ELISA Kit
Product Code:HUDL01565
Size:96 Assays
Target:ITGa2
Synonyms:CD49b, BR, GPIa, VLA-2, VLAA2, Platelet membrane glycoprotein Ia, Alpha 2 Subunit Of VLA-2 Receptor, Very Late Activation Proteins 2 Receptor, Collagen receptor, VLA-2 alpha
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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